Considerations Regarding CHSY1 Knockdown and Its Influence on Gene Expression Profiles: A Perspective from Protein Interaction Network Analysis

Authors

  • Mona Zamanian-Azodi Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Author
  • Alireza Ahmadzadeh Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of lablatory Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Author
  • Mitra Rezaei Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Author
  • Reza M Robati Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Author

DOI:

https://doi.org/10.31557/apjcb.2024.9.3.323-328

Keywords:

Ovarian Cancer (OC), CHSY1 knockdown, Gene Expression, Network analysis

Abstract

Introduction: Knockdown of CHSY1 in ovarian cancer holds promise for monitoring tumorigenesis and the application of targeted therapies.

Method: A comparison was made between microarray datasets of ovarian cancer with and without CHSY1 knockdown to identify differentially expressed genes (DEGs). Subsequently, the DEGs were analyzed using Cytoscape and its plug-ins. All key genes in the protein-protein interaction (PPI) network originated from the expression dataset which exhibiting significant differential expressions.

Result: Particularly PTEN, STAT3, BRCA, CCND1, and HIST1H4I emerged as central hub-bottlenecks within the PPI network following CHSY1 knockdown.

Conclusion: This compilation suggests that the candidate genes and their associated biological processes may have substantial connections to the effects of knockdown, inhibiting some cancer-related genes to prevent cancer progression.

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Published

2024-09-01

Issue

Vol. 9 No. 3 (2024)

Section

Research Articles/ Original Work

License

 West Asia Organization for Cabcer Prevention retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License 4 (This permits anyone to copy, distribute, transmit and adapt the published work, provided the original work and source are appropriately cited).